UA 2301 then AA 5912 at Charlotte
Newark → Charlotte → Tri-Cities
Timetable, Charlotte time
22:13UA 2301 from Newark at the gate
21:54AA 5912 to Tri-Cities leaves the gate
23 h 41 apart on the timetable, overnight
AA 5912 leaves 23 h 41 after UA 2301 arrives on the timetable: more than half a day, so not a connection we count.