DL 3854 then DL 4159 at Detroit
Green Bay → Detroit → Appleton
Timetable, Detroit time
20:31DL 3854 from Green Bay at the gate
20:00DL 4159 to Appleton leaves the gate
23 h 29 apart on the timetable, overnight
DL 4159 leaves 23 h 29 after DL 3854 arrives on the timetable: more than half a day, so not a connection we count.