AA 110 then FR 5316 at Fiumicino
Chicago → Rome → Madrid
Timetable, Rome time
08:55AA 110 from Chicago at the gate
05:45FR 5316 to Madrid leaves the gate
20 h 50 apart on the timetable, overnight
FR 5316 leaves 20 h 50 after AA 110 arrives on the timetable: more than half a day, so not a connection we count.