AZ 1620 then FR 5316 at Fiumicino
Brindisi → Rome → Madrid
Timetable, Rome time
07:35AZ 1620 from Brindisi at the gate
05:45FR 5316 to Madrid leaves the gate
22 h 10 apart on the timetable, overnight
FR 5316 leaves 22 h 10 after AZ 1620 arrives on the timetable: more than half a day, so not a connection we count.