FR 6927 then W4 6065 at Fiumicino
Barcelona → Rome → Prague
Timetable, Rome time
15:00FR 6927 from Barcelona at the gate
12:40W4 6065 to Prague leaves the gate
21 h 40 apart on the timetable, overnight
W4 6065 leaves 21 h 40 after FR 6927 arrives on the timetable: more than half a day, so not a connection we count.