FR 8561 then FR 5842 at Fiumicino
Valletta → Rome → Brindisi
Timetable, Rome time
15:15FR 8561 from Valletta at the gate
14:50FR 5842 to Brindisi leaves the gate
23 h 35 apart on the timetable, overnight
FR 5842 leaves 23 h 35 after FR 8561 arrives on the timetable: more than half a day, so not a connection we count.