UA 3496 then AA 5332 at Dulles
Jacksonville → Washington → Charlotte
Timetable, Washington time
13:35UA 3496 from Jacksonville at the gate
10:44AA 5332 to Charlotte leaves the gate
21 h 09 apart on the timetable, overnight
AA 5332 leaves 21 h 09 after UA 3496 arrives on the timetable: more than half a day, so not a connection we count.