UA 2197 then TK 134 at Houston
Pittsburgh → Houston → Istanbul
Timetable, Houston time
15:11UA 2197 from Pittsburgh at the gate
15:05TK 134 to Istanbul leaves the gate
23 h 54 apart on the timetable, overnight
TK 134 leaves 23 h 54 after UA 2197 arrives on the timetable: more than half a day, so not a connection we count.