TK 2143 then TK 254 at Istanbul
Ankara → Istanbul → Dushanbe
Timetable, Istanbul time
20:25TK 2143 from Ankara at the gate
19:10TK 254 to Dushanbe leaves the gate
22 h 45 apart on the timetable, overnight
TK 254 leaves 22 h 45 after TK 2143 arrives on the timetable: more than half a day, so not a connection we count.