BA 541 then AA 735 at Heathrow
Bologna → London → Charlotte
Timetable, London time
14:30BA 541 from Bologna at the gate
14:10AA 735 to Charlotte leaves the gate
23 h 40 apart on the timetable, overnight
AA 735 leaves 23 h 40 after BA 541 arrives on the timetable: more than half a day, so not a connection we count.