BA 581 then AA 735 at Heathrow
Milan → London → Charlotte
Timetable, London time
14:35BA 581 from Milan at the gate
14:10AA 735 to Charlotte leaves the gate
23 h 35 apart on the timetable, overnight
AA 735 leaves 23 h 35 after BA 581 arrives on the timetable: more than half a day, so not a connection we count.