FR 5300 then FR 2436 at Madrid
Luxembourg → Madrid → Rome
Timetable, Madrid time
20:00FR 5300 from Luxembourg at the gate
18:35FR 2436 to Rome leaves the gate
22 h 35 apart on the timetable, overnight
FR 2436 leaves 22 h 35 after FR 5300 arrives on the timetable: more than half a day, so not a connection we count.