FR 5316 then FR 2766 at Madrid
Rome → Madrid → Prague
Timetable, Madrid time
23:40FR 5316 from Rome at the gate
18:20FR 2766 to Prague leaves the gate
18 h 40 apart on the timetable, overnight
FR 2766 leaves 18 h 40 after FR 5316 arrives on the timetable: more than half a day, so not a connection we count.