FR 9471 then FR 2766 at Madrid
Pisa → Madrid → Prague
Timetable, Madrid time
20:00FR 9471 from Pisa at the gate
18:20FR 2766 to Prague leaves the gate
22 h 20 apart on the timetable, overnight
FR 2766 leaves 22 h 20 after FR 9471 arrives on the timetable: more than half a day, so not a connection we count.