MQ 4638 then AA 2541 at Miami
Tampa → Miami → Houston
Timetable, Miami time
20:51MQ 4638 from Tampa at the gate
19:57AA 2541 to Houston leaves the gate
23 h 06 apart on the timetable, overnight
AA 2541 leaves 23 h 06 after MQ 4638 arrives on the timetable: more than half a day, so not a connection we count.