FR 1644 then VF 335 at Vienna
Rome → Vienna → Prague
Timetable, Vienna time
21:30FR 1644 from Rome at the gate
13:40VF 335 to Prague leaves the gate
16 h 10 apart on the timetable, overnight
VF 335 leaves 16 h 10 after FR 1644 arrives on the timetable: more than half a day, so not a connection we count.